ABSTRACT
Parasitosis are the most common diseases in the world, they are responsible for relevant negative consequences in the individuals´ health. The consumption of vegetables in Brazil and in the world is common due to their high nutritional value and they are recommended to be included in the population´s diet. Contaminations in vegetables are most often caused by irrigation water which is contaminated by feces, other forms of contamination are through organic manure containing fecal waste and the contact of vegetables with animals where they are grown. This research evaluated the contamination in vegetables with the purpose of warning the community about hygiene and the correct approach of food handling in order to prevent parasitic infections, contributing to people´s health. Samples were collected from supermarkets and farmers´ markets, where we obtained a total of 30 samples that were analyzed using Hoffman, centrifugation and slide reading´s techniques. In all samples, parasites were found. In six samples non-pathogenic amoebas were found, however this finding means that there was contamination from human or animal feces. And, in the other 24 samples, parasitic structures that cause damage to health were found. This work showed a high level of contamination of the vegetables that are consumed daily by the population and the diseases caused by food which are a concern for the public health. Contaminated vegetables are dangerous considering the transport of parasites that may generate human infections, therefore the importance to create public policies in order to avoid parasitic infections.
Subject(s)
Parasitic Diseases , Parasitology , Vegetables , Food Contamination , Neglected Diseases , Food , Food Handling , SupermarketsABSTRACT
Listeria monocytogenes é um patógeno relevante veiculado por alimentos. Sua identificação precisa é importante para a correta determinação do risco associado à ingestão do alimento. O objetivo deste trabalho foi verificar a ocorrência das espécies de Listeria spp. em amostras de salsichas tipo hot dog e carne moída bovina, comercializadas a granel no Distrito Federal. Foram analisadas 162 amostras, sendo 127 de salsichas tipo hot dog e 35 amostras de carne moída bovina. O isolamento e a identificação do gênero foram feitos por metodologia convencional e a distinção das espécies foi verificada por kit bioquímico específico (API-Listeria®) e por análise de restrição de fragmentos da reação em cadeia da polimerase (RFLP-PCR) do gene 23 rRNA. Foram isoladas 26 cepas de Listeria spp. das amostras de salsichas tipo hot dog, sendo identificadas 18 cepas de Listeria innocua e 08 cepas de Listeria monocytogenes. Das 35 amostras de carne moída bovina, foram isoladas 16 cepas de Listeria spp., sendo identificadas 12 cepas de Listeria innocua e 04 de Listeria monocytogenes. Houve concordância total na distinção das espécies de Listeria spp. através dos dois métodos empregados. A presença de Listeria spp. em amostras de salsicha do tipo hot dog a granel e em carne moída bovina a granel, de estabelecimentos comerciais do Distrito Federal, representa risco à saúde do consumidor.
Listeria monocytogenes is a pathogen disclosed by foods. Its accurate identification is important for the correct determination of the risk associated with the ingestion of food. The aim of this research was to determine the occurrence of Listeria spp. in samples of hot dog sausages and ground beef, sold in bulk in the Federal District. A total of 162 samples, 127 hot dog sausages and 35 samples of ground beef cattle, were analyzed. The isolation and identification of the genus were made by conventional methodology and distinction of species was verified by specific biochemical kit (API-Listeria®) and by restriction fragment length polymorphism (PCR-RFLP) of 23S rRNA gene. Twenty-six strains of Listeria spp. were isolated. Samples of hot dog sausages identified 18 strains of Listeria innocua and 08 strains of Listeria monocytogenes. Of the 35 samples of ground beef cattle were isolated, 16 strains of Listeria spp., 12 strains of Listeria innocua and 04 strains of Listeria monocytogenes. There was total agreement in the distinction of Listeria species using the two methods. The presence of Listeria spp. in samples of hot dog sausages and ground beef, sold in bulk in commercial establishment of the Federal District represents a risk to consumer health.